Microfibrillar-associated protein 5 is a 25kD microfibril-associated glycoprotein which is rich in serine and threonine residues.
It lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31kD microfibril-associated glycoprotein (MFAP2).
The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs.
The structural differences suggest that this encoded protein has some functions that are distinct from those of MFAP2.MAGP2 lacks the proline-, glutamine-, and tyrosine-rich sequences and a hydrophobic carboxyl terminus, characteristic of MAGP1.
These structural differences suggest that MAGP2 has some functions that are distinct from those of MAGP1.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat MFAP5.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Asp32-Asp164 of rat MFAP5, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
