Microfibrillar-associated protein 2 is a major antigen of elastin-associated microfibrils and a candidate for involvement in the etiology of inherited connective tissue diseases.
This gene encodes two transcripts with two alternatively spliced 5' untranslated exons.
These two transcripts contain the same 8 coding exons, and therefore, encode the same protein
Chen et al.
(1993) demonstrated that the mouse Magp transcript is a widespread product of mesenchymal/connective tissue cells throughout mouse development.
A human MFAP2 cDNA was cloned by Faraco et al.
(1995) from a human lung cDNA library using a human probe initially identified with primers from the bovine MFAP2 gene.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size:200ul
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes rat MFAP2.
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Rt
Immunogen:Recombinant protein corresponding to Leu8-Cys17 of rat MFAP2, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.