METTL21C Belongs to the methyltransferase superfamily.
METTL21 family.
A methyltransferase is a type of transferase enzyme that transfers a methyl group from a donor to an acceptor.
Methylation often occurs on nucleic bases in DNA or amino acids in protein structures.
Methytransferases use a reactive methyl group bound to sulfur in S-adenosyl methionine (SAM) as the methyl donor.DNA methylation is often utilized to silence and regulate genes without changing the original DNA sequence, an example of epigenetic modification.
This methylation occurs on cytosine residues.
DNA methylation may be necessary for normal growth from embryonic stages in mammals.
When mutant embryonic stem cells lacking the murine DNA methyltransferase gene were introduced to a germline of mice, they caused a recessive lethal phenotype.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat METTL21C.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Met1-Asp248 of rat METTL21C, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
