Meteorin regulates glial cell differentiation and promotes the formation of axonal networks during neurogenesis.
The deduced 290aa protein shares 82% amino acid identity with the 293aa human ortholog.
Both human and mouse meteorin contain a putative N-terminal signal peptide.
Immunoblot analysis of cell fractions from Chinese hamster ovary cells expressing mouse meteorin localized meteorin to the lysate and culture supernatant, suggesting that meteorin is secreted.
In situ hybridization of developing mouse tissues showed predominant meteorin expression in the central and peripheral nervous system in neural progenitors, glial progenitors, and cells of the astrocyte lineage.
Meteorin induction of neurite outgrowth showed an additive effect with neurotrophin-3 (NTF3) but not with NGF (NGFB) or BDNF.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat METRN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Gly22-Asp29 of rat METRN, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
