Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes and in leukocytes and certain cancer cells.
Mature meprins are oligomers of evolutionarily related, separately encoded alpha and/or beta subunits.
Homooligomers of meprin-alpha (MEP1A) are secreted; oligomers containing meprin-beta are associated with the plasma membrane
Mep1b-null mice were born at half the expected mendelian ratio, but those that survived developed normally and were viable and fertile.
Mutant mice lacked membrane-associated Mep1a in kidney and intestine, and microarray analysis indicated that renal gene expression profiles were different than those of wildtype mice.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat MEP1b.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Trp433-Tyr679 of rat MEP1b, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
