Meprins, members of the 'astacin family' of metalloendopeptidases, are capable of hydrolyzing a variety of peptide and protein substrates.
Two forms of meprins, designated A and B.
Meprin A (EC 3.4.24.18) is the best characterized of the meprins and is known to hydrolyze peptides such as bradykinin, melanocyte-stimulating hormone, neurotensin, luteinizing hormone releasing hormone, transforming growth factor-alpha, and parathyroid hormone.
Meprin B from mouse kidney has latent proteolytic activity against polypeptide substrates.
Rat meprin A is sometimes referred to as endopeptidase-2 or endopeptidase-24.18; human meprin A is also called N-benzoyl-L-tyrosyl-p-aminobenzoic acid (PABA peptide) hydrolase (PPH).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MEP1a.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to His213-Val506 of human MEP1a, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
