Myocyte-specific enhancer factor 2A is a transcription factor in the Mef2 family.
In humans it is located on chromosome 15q26.
Certain mutations in MEF2A cause an autosomal dominant form of coronary artery disease and myocardial infarction.The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression.
The myogenic basic helix-loop-helix proteins, including myoD, myogenin, MYF5, and MRF4 are 1 class of identified factors.
A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family.
Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse MEF2A.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Ser160 of mouse MEF2A, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
