In both leukocytes and placentas, Davidson and Cortner (1967) found polymorphism of the malate dehydrogenase that is bound to mitochondria, called M-MDH originally and now symbolized MDH2.
The fact that mitochondrial malate dehydrogenase was indistinguishable from normal in persons with variation in the supernatant MDH indicates that a separate locus is involved in its genetic determination.
Mendelian segregation rather than maternal inheritance of MDH2 suggests that not all mitochondrial proteins are coded by mitochondrial DNA.
Mitochondrial glutamic oxaloacetic transaminase is also determined by nuclear genes.
From study of hybrid cells, Van Heyningen et al.
(1975) mapped the locus for MDH2 to chromosome 7.
Habets et al.
(1992) subsequently mapped the locus to 7cen-q22.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MDH2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala25-Lys338 of human MDH2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
