Malondialdehyde, MDA, is a highly reactive three carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation and arachidonic acid metabolism.
MDA readily combines with several functional groups on molecules including proteins, lipoproteins, and DNA.
MDA-modified proteins may show altered physico-chemical behavior and antigenicity. Malondialdehyde is a highly reactive compound that is not typically observed in pure form.
In the laboratory it can be generated in situ by hydrolysis of 1,1,3,3- tetramethoxypropane, which is commercially available.
It is easily deprotonated to give the sodium salt of the enolate (m.p.
245°C).
Malondialdehyde reacts with deoxyadenosine and deoxyguanosine in DNA, forming DNA adducts, primarily M1G, which is mutagenic.
The guanidine group of arginine residues condense with MDA to give 2-aminopyrimidines.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes MDA. Species Crossreactivity: pan-species (general)
