Induced myeloid leukemia cell differentiation protein Mcl-1 is a protein belongs to the Bcl-2 family.
Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.
The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation.
Mediates its effects by interactions with a number of other regulators of apoptosis.
Isoform 1 inhibits apoptosis while isoform 2 promotes it.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse MCL1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Phe2-Gly307 of mouse MCL1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
