The deduced 1,265aa protein shares significant similarity with mouse Mast205.
RT-PCR ELISA detected variable MAST2 expression in all tissues examined.
The deduced 1,734aa protein has a calculated molecular mass of 190.5kD.
It had an apparent molecular mass of 205kD by SDS-PAGE.
Mast205 contains a central serine/threonine kinase catalytic domain, which includes an ATP-binding consensus sequence, and several potential phosphorylation sites.
Northern blot analysis of mouse tissues detected Mast205 expression only in testis.
Mast205 expression increased during postnatal testicular development coincident with meiosis/spermiogenesis.
Immunofluorescence microscopy showed Mast205 associated with mouse spermatid manchette.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MAST2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to Phe512-Phe785 of human MAST2, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
