MAP3K6 is a member of the serine/threonine protein kinase family.
The encoded kinase was identified by its interaction with MAP3K5/ASK, a protein kinase and an activator of c-Jun kinase (MAPK7/JNK) and MAPK14/p38 kinase.
This kinase was found to weakly activate MAPK7, but not MAPK1/ERK or MAPK14.
An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined
MAP3K6 and MAP3K5 share 45% aa sequence identity; their catalytic domains share 82% identity.
The authors confirmed the interaction of MAP3K6 with MAP3K5 in vivo using coimmunoprecipitation.
Recombinant MAP3K6 weakly activated the JNK but not the ERK or p38 kinase pathways.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat MAP3K6.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Thr649-Leu907 of rat MAP3K6 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
