Myelin-associated glycoprotein (MAG) is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR MAG is believed to be involved in myelination during nerve regeneration.
Myelin-associated glycoprotein (MAG), a membrane protein of 100kD, is thought to be involved in the process of myelination (Barton et al., 1987).
MAG is a sialic acid-binding SIGLEC protein and is a functional ligand for the NOGO receptor (NGR, or RTN4R) (Liu et al., 2002).
Spagnol et al.
(1989) found that MAG cDNA clones isolated from a human brain cDNA library had an open reading frame of 1878 nucleotides encoding a peptide of 626aa with a calculated molecular weight of 69.1kD.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MAG.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Lys328-Ile514 of human MAG fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
