84-4426-32　LXN (Latexin, ECI, TCI, MUM, Endogenous carboxypeptidase inhibitor, Tissue carboxypeptidase inhibitor) 200ul　364026

特徴

• Latexin is a specific inhibitor of zinc-dependent metallocarboxypeptidases.
• The deduced 222aa protein is an elongated molecule with N- and C-terminal domains that each consist of an alpha helix enveloped by a curved beta sheet.
• The 2 domains are separated by a connecting segment.Lxn was less potent against other mammalian carboxypeptidases, and it did not inhibit other metallopeptidases or serine proteases
• It also showed low specificity, inhibiting several metallocarboxypeptidases containing a characteristic alpha/beta hydrolase fold.
• The inhibition constant was within the nanomolar range for these substrates.
• LXN did not inhibit enzymes of other carboxypeptidase classes.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MGHHHHHHSG SEF- MEEIPPTHYA ASRAASVAEN CINYQQGTPN KVFKVQTVQQ ASKEDIPGRG HKYHLKFSVE EIIQKQVTVS CTAEVLYPRM GQGSAPEVNF TFEGEIGKNP DEEDNTFYQR LMSMKEPLQA QNIPDNFGNV SPQMKPVHHL AWVACGYVMW QNSTEDTWYK MAKIQTVKQV QRNDDFIELD YTVLLHDVAS QEIIPWQMQV LWHPQYGTKV KHNSRLPKEA PAE
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.