LTF, a globular multifunctional protein with antimicrobial activity (bacteriocide, fungicide), is part of the innate defense, mainly at mucoses.
Lactoferrin is found in milk and many mucosal secretions such as tears and saliva.
Lactoferrin is also present in secondary granules of PMN and also is secreted by some acinar cells.
Human colostrum has the highest concentration, followed by human milk, then cow milk.
Lactoferrin belongs to the transferrin family proteins (TF, melanotransferrin, ovotransferin, etc.).
Its molecular mass is 80,000 u (80kD).
Lactoferrin antimicrobial activity is due partly to its high affinity for Fe (ferric state).
LF proteolysis produces lactoferricin, kaliocin-1 small peptides with antimicrobial activity.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat LTF.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Cys64-Gly193 of rat LTF, fused to His-Tag at N-terminal expressed in E. coli.
