LRRN6A encodes a transmembrane leucine-rich repeat protein, LERN1with similarity to proteins involved in axonal guidance and migration, nervous system development and regeneration processes.
The identification of homologous genes to LRRN6A on chromosomes 9 and 19 and the orthologous genes in the mouse genome and other organisms suggests that LERN proteins constitute a novel subfamily of LRR (leucine-rich repeat)-containing proteins.
The LRRN6A expression pattern is specific to the central nervous system, highly and broadly expressed during early stages of development and gradually restricted to forebrain structures as development proceeds.
Expression level in adulthood is lower in general but remains stable and significantly enriched in the limbic system and cerebral cortex.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunocytochemistry: 5-20ug/mlImmunohistochemistry (Frozen): 5-20ug/mlImmunohistochemistry (Paraffin): 5-20ug/mlOptimal dilutions to be determined by the researcher.
Included Protein Molecular Weight Marker:168631: Unstained Protein Molecular Weight Marker, 10-70kDSuppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol.
Ready to use; no need to heat, dilute or add reducing agents before use.
Protein Bands: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD
Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier.
Usage: Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20°C will go back into solution.
Do not boil! Load the following volumes on SDS-PAGE gel:Mini gels: 3-5ul/wellLarge gel: 7ul/wellThe loading volumes listed above are recommended for gels with thickness of 0.75-1mm.
The loading volume should be doubled for 1.5mm thick gels
Store at -20°C Stable for 6 months after receip
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% Proclin-300, 50% glycerol.
Specificity：Recognizes human LRRN1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser418-Ala631 of human LRRN1 expressed in E. coli.
