A leucine-rich repeat (LRR) is a protein structural motif that forms an alpha/beta horseshoe fold.
It is composed of repeating aa20-30 stretches that are unusually rich in the hydrophobic aa acid leucine.
Typically, each repeat unit has beta strand-turn-alpha helix structure, and the assembled domain, composed of many such repeats, has a horseshoe shape with an interior parallel beta sheet and an exterior array of helices
In an effort to isolate as many candidate oncogenes as possible from the region 11q13.5-q14, Ollendorff et al.
(1992) isolated a sequence, containing a gene they provisionally named GARP.
They cloned the homologous sequence in the mouse and located it on chromosome 7.
The murine and human genes were mapped by in situ hybridization.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat LRRC32.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Leu162-Tyr405 of rat LRRC32 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
