Leupaxin is preferentially expressed in hematopoietic cells and is most homologous to the focal adhesion protein, paxillin.
It may function in cell type-specific signaling by associating with PYK2.
As a substrate for a tyrosine kinase in lymphoid cells, this protein may also function in, and be regulated by tyrosine kinase activity.
LPXN shares 37% overall aa sequence identity with the focal adhesion protein paxillin (PXN).
The C terminus of LPXN contains 4 LIM domains that share 67 to 76% identity with the LIM domains of PXN.
Its N terminus contains 3 LD motifs, which were first identified in PXN as 13aa motifs with an invariable leucine and aspartate pair.
LD motifs in PXN have been implicated in binding to cytoplasmic PTK2 and vinculin, resulting in localization of PTK2 to focal adhesions.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry:: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse LPXN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Ser208 of mouse LPXN, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
