Lysophosphatidylcholine (LPC) acyltransferase (LPCAT) catalyzes the conversion of LPC to phosphatidylcholine (PC) in the remodeling pathway of PC biosynthesis deduced 534aa mouse and human proteins contain 3 transmembrane domains, several motifs conserved in members of the LPCAT family, a putative EF hand domain, and a C-terminal endoplasmic reticulum (ER) retention signal.
They share about 88% aa identity.
Real-time RT-PCR of mouse tissues showed that Lpcat1 was expressed at highest levels in lung, followed by spleen and brain.
Other tissues had low Lpcat1 levels.
Confocal microscopy detected epitope-tagged Lpcat1 mainly in the ER and Golgi of transfected cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse LPCAT1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ala301-Cys514 of mouse LPCAT1 fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
