Lipoprotein(a) consists of an LDL-like particle and the specific apolipoprotein(a), which is covalently bound to the apoB of the LDL like particle.
Lp(a) plasma concentrations are highly heritable and mainly controlled by the apolipoprotein(a) genelocated on chromosome 6q26-27.
Apo(a) proteins vary in size due to a size polymorphism, which is caused by a variable number of so called kringle IV repeats in the LPA gene.
This size variation at the gene level is expressed on the protein level as well, resulting in apo(a) proteins with 10 to > 50 kringle IV repeats.
These variable apo(a) sizes are known as "apo(a) isoforms".
There is a general inverse correlation between the size of the apo(a)isoform and the Lp(a) plasma concentration which is caused by a variable rate of degradation before the apo(a) protein has matured for Lp(a) assembly.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Lpa.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asp4227-Arg4546 of human Lpa fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
