By searching an EST database for sequences similar to dog Vip36, Neve et al.
(2003) identified LMAN2, which they called VIP36.
The deduced 356aa protein contains an N-terminal signal sequence, followed by a lectin-type carbohydrate recognition domain and a C-terminal transmembrane domain.
A KRFY endoplasmic reticulum trafficking motif is located at the C-terminal.
Northern blot analysis detected variable expression of 1.2- and 1.4kb VIP36 transcripts in all tissues examined.
Highest expression was in kidney, placenta, and liver
Nufer et al.
(2003) determined that the LMAN2 gene contains 8 exons and spans 14.9kb.
By genomic sequence analysis, Nufer et al.
(2003) mapped the LMAN2 gene to chromosome 5q35.5.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human LMAN2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asp45-Arg322 of human LMAN2 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
