Lipoprotein lipase (EC 3.1.1.34) is an enzyme which hydrolyzes lipids in lipoproteins, like those found in chylomicrons and very low density lipoproteins (VLDL), into three free fatty acids and one glycerol molecule.
It requires Apo-CII as a cofactor.
Lipoprotein lipase is specifically found in endothelial cells lining the capillaries
Insulin is known to enhance LPL synthesis in adipocytes and its placement in the capillary endothelium.
LPL has different isozymes in different tissues.
The form that is in adipocytes is activated by insulin, whereas that in muscle and myocardium is not.
This helps to explain why adipose cells gain fat in a well-fed state.
A number sign is used with this entry because type I hyperlipoproteinemia can be caused by mutation in the lipoprotein lipase gene (LPL).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat LIPD.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ala28-Gly474 of rat LIPD fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
