LIMCH1 contains one CH (calponin-homology) domain and one LIM zinc-binding domain.
There are nine named isoforms.
The calponin homology (CH) domain is a protein module of about 100 residues that was first identified at the N-terminal of calponin, an actin-binding protein playing a major regulatory role in muscle contraction.
Three major groups of CH-domain-containing proteins have been recognized on the basis of sequence analysis.
Proteins containing a single N-terminal CH domain (1xCH proteins) include calponin itself as well as signaling proteins such as Vav, IQGAP and Cdc24.
Proteins with an F-actin-binding domain (ABD) composed of two CH domains in tandem (2xCH proteins) include spectrins, dystrophin, filamins and plakins.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse LIMCH1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Glu21-Asn125 of mouse LIMCH1 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
