84-4425-70　LIG1 (ATP Dependent DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1) 200ul　363966

特徴

• The major DNA ligase activity in proliferating mammalian cells is due to the high molecular weight enzyme designated DNA ligase I.
• Barnes et al.
• (1990) cloned cDNA for human DNA ligase I, both by functional complementation of a Saccharomyces cerevisiae cdc9 mutation and by hybridization with oligonucleotide probes corresponding to peptide sequences of the purified bovine enzyme.
• Hybridization between the cloned sequences and mRNA as well as genomic DNA indicated that the human enzyme is transcribed from a single-copy gene.
• Barnes et al.
• (1990) mapped the DNA ligase I gene to chromosome 19 by hybridization to DNAs from well-characterized rodent-human somatic cell hybrids retaining subsets of human chromosomes.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KYY GQRAQIHVLE GGEVKIFSRN QEDNSGKYPD IISRIPKIKH PSVTSFILDT EAVAWDREKK QIQPFQVLTT RKRKEVDASE IQVQVCLYAF DLIYLNGESL ARQPLSRRRQ LLRENFVETE GEFVFATSLD TKDIEQIAEF LEQSVKDSCE GLMVKTLDVD ATYEIAKRSH NWLKLKKDYL EGVGDTLDLV VIGAYLGRGK RPGRYGGFLL AAYDEESEEL AAICKLGTGF SDEELEEHHQ NMQALLLPTP RPYVRIDGAV APNHWLDPSI VWEVKCADLT LSPIYRAARG MVDKEKGISL RFPRF
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.