84-4425-69　LIG1 (ATP Dependent DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1) 200ul　363965

特徴

• The major DNA ligase activity in proliferating mammalian cells is due to the high molecular weight enzyme designated DNA ligase I.
• Barnes et al.
• (1990) cloned cDNA for human DNA ligase I, both by functional complementation of a Saccharomyces cerevisiae cdc9 mutation and by hybridization with oligonucleotide probes corresponding to peptide sequences of the purified bovine enzyme.
• Hybridization between the cloned sequences and mRNA as well as genomic DNA indicated that the human enzyme is transcribed from a single-copy gene.
• Barnes et al.
• (1990) mapped the DNA ligase I gene to chromosome 19 by hybridization to DNAs from well-characterized rodent-human somatic cell hybrids retaining subsets of human chromosomes.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.