Legumain is a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds.
This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems.
Enzyme activation is triggered by acidic pH and appears to be autocatalytic.
Protein expression occurs after monocytes differentiate into dendritic cells.
A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells.
Overexpression of this gene may be associated with the majority of solid tumor types.
This gene has a pseudogene on chromosome 13.
Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human LGMN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Val18-Tyr433 of human LGMN fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
