The deduced 549aa protein contains a N-terminal signal peptide, a putative transmembrane region, and 1 partial and 4 complete leucine-rich repeats (LRRs).
The LRRs are flanked on both sides by cysteine-rich regions, and the N-terminal cysteine-rich region contains a motif conserved in LGIs.
LGI3 shares significant similarity with LGI1, LGI2, and LGI4.
Semiquantitative PCR detected LGI3 expression in all tissues examined, with highest levels in brain and lung
By in situ hybridization of adult mouse brain, Senechal et al.
(2005) showed that Lgi3 was expressed at low levels in most brain regions with high levels in the facial nerve nucleus.
Lgi3 was secreted in transfected 293T cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human LGI3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Lys261-Ala548 of human LGI3 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
