Leucine is a branched-chain alpha-amino acid with the chemical formula HO2CCH(NH2)CH2CH(CH3)2.
It is an essential amino acid, which means that humans cannot synthesize it.
Its codons are UUA, UUG, CUU, CUC, CUA, and CUG.
With a hydrocarbon side chain, leucine is classified as a hydrophobic amino acid.
It has an isobutyl R group.
Leucine is a major component of the sub units in ferritin, astacin and other 'buffer' proteins.
Leucine is utilized in the liver, adipose tissue, and muscle tissue.
In adipose and muscle tissue, leucine is used in the formation of sterols, and the combined usage of leucine in these two tissues is seven times greater than its use in the liver.
Leucine is the only dietary protein that has the capacity to stimulate muscle protein synthesis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes Leu. Species Crossreactivity: pan-species (general)
