Monocyte/neutrophil elastase inhibitor (EI) is a protein of approximately 42,000 Mr with serpin-like functional properties.
Remold-O'Donnell et al.
(1992) cloned EI cDNA and identified 3 EI mRNA species of 1.5, 1.9, and 2.6kb in monocyte-like cells and no hybridizing mRNA in lymphoblastoid cells lacking detectable EI enzymatic activity.
The cDNA open reading frame encoded a 379aa protein.
Its sequence established EI as a member of the serpin superfamily.
Sequence alignment indicated that the reactive center P1 residue is cys-344, consistent with abrogation of elastase inhibitory activity by iodoacetamide and making EI a naturally occurring cys-serpin.
Other elastase inhibitors include alpha-1-antitrypsin (PI) and secretory leukocyte protease inhibitor (SLPI).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
