Lactase, a part of the beta-galactosidase family of enzymes, is a glycoside hydrolase involved in the hydrolysis of the disaccharide lactose into constituent galactose and glucose monomers.
Lactase is present predominantly along the brush border membrane of the differentiated enterocytes lining the villi of the small intestine.
In humans, lactase is encoded by the LCT gene.
Lactase is essential for digestive hydrolysis of lactose in milk.
Deficiency of the enzyme causes lactose intolerance.
The optimum temperature for lactase is about 25 C for its activity and has an optimum pH of 6.Lactase can hydrolyze a variety of substrates.
While it is most notably a member of the beta-galactosidase enzymatic class, lactase also has glucosidase and glycosylceramidase activity.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat LCT.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ala33-Ala291 of rat LCT fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
