The lipocalins are a family of proteins which transport small hydrophobic molecules such as steroids, bilins, retinoids, and lipids.
They share limited regions of sequence homology and a common tertiary structure architecture.
This is an eight stranded antiparallel beta-barrel with a repeated + 1 topology enclosing an internal ligand binding site
These proteins are found in gram negative bacteria, vertebrate cells, and invertebrate cells, and in plants.
Lipocalins have been associated with many biological processes, among them immune response, pheromone transport, biological prostaglandin synthesis, retinoid binding, and cancer cell interactions.
The name "lipocalin" has been proposed for this protein family, but cytosolic fatty acid binding proteins are also included.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes rat LCNL1.
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Rt
Immunogen:Recombinant protein corresponding to Val2-Leu164 of rat LCNL1 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.