Leucine aminopeptidase (LAP) is an exopeptidase hydrolyzing the peptide bond adjacent to a free amino group.
LAP is extensively used in the sequence analysis of proteins and peptides.
The enzyme liberates aa’s from the N-terminal end of a number of proteins and polypeptides.
It is called leucine aminopeptidase because it reacts most rapidly with leucine compounds.
Many aliphatic amides are also hydrolyzed as well as thiolesters.
LAP consists of four subunits each having one atom of zinc
PEPS levels did not differ from controls in any.
This experience permitted exclusion mapping of PEPS to 4p11-4q13; combined with previously reported data, the assignment becomes 4p11-4q12.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat LAP.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Pro206-Arg496 of rat LAP fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
