By screening a DC subtraction library, de Saint-Vis et al.
(1998) isolated a cDNA encoding DCLAMP.
Sequence analysis predicted that the 416aa type I integral membrane protein has an N-terminal signal peptide, followed by a 381aa extracellular domain, a hydrophobic transmembrane domain, and a 10aa cytoplasmic domain containing a conserved GY lysosomal targeting motif, characteristic of LAMP family members
The DCLAMP protein contains multiple N- and O-glycosylation sites.
Northern blot and RT-PCR analysis revealed expression of a 3.2kb transcript in appendix, thymus, lymph node, lung, and weakly in spleen, as well as in dendritic cell lines; expression was weak or undetectable in other cell lines.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human LAMP3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser222-Thr381 of human LAMP3 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
