LAMB3 is a laminin that belongs to a family of basement membrane proteins.
This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5.
Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin.
Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene.
LAMb3 is approximately 29kb long and consists of 23 exons that vary from 64 to 379 basepairs, accounting for the full-length cDNA with an open reading frame of 3516bp encoding 1172 aa.
In comparison with the LAMB1 gene, LAMB3 is considerably more compact.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat LAMb3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Cys503-Ser738 of rat LAMb3 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
