Laminin subunit beta-2 is the beta chain isoform laminin, beta 2.
The beta 2 chain contains the 7 structural domains typical of beta chains of laminin, including the short alpha region.
However, unlike beta 1 chain, beta 2 has a more restricted tissue distribution.
It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle.
Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination, showed defects in the maturation of neuromuscular junctions and impairment of glomerular filtration.
Alternative splicing involving a non consensus 5' splice site (gc) in the 5' UTR of this gene has been reported.
It was suggested that inefficient splicing of this first intron, which does not change the protein sequence, results in a greater abundance of the un.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat LAMb2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Gln939-Ser1129 of rat LAMb2 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
