Laminin is a basement membrane protein composed of 3 nonidentical chains, A (400kD), B1 (230kD), and B2 (220kD), which are arranged in a cross-shaped structure.
Mattei et al.
(1989) used in situ hybridization to localize the human laminin A locus to chromosome 18p11.31.
By study of mouse-Chinese hamster somatic cell hybrids, Kaye et al.
assigned the gene for laminin A to mouse chromosome 17 and confirmed the assignment of the gene for laminin B2 to mouse chromosome 1.
Haaparanta et al.
reported the nucleotide sequence of LAMA cDNA.
They found an open reading frame encoding 3075aa.
Nissinen et al.
reported that the complete human laminin A chain contains a 17aa signal peptide and a 3058-residue A chain proper.
The chain has a distinct domain structure with numerous internal cysteine-rich repeats.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat LAMa1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Pro1156-Ile1276 of rat LAMa1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
