Lymphocyte-activation protein 3 belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains.
The LAG3 gene contains 8 exons.
The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4.
Triebel et al.
(1990) identified a novel human gene of the Ig superfamily, which they designated LAG3.
Expression of this gene was undetectable in resting peripheral blood lymphocytes, whereas it was found in activated T and NK cells.
The corresponding cDNA encodes a 498aa membrane protein with 4 extracellular Ig-like domains.
The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4.
The 2 genes are probably derived from a common evolutionary ancestor.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat LAG3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Asn209-Leu451 of rat LAG3 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
