Ladinin-1 may be an anchoring filament that is a component of basement membranes.
It may contribute to the stability of the association of the epithelial layers with the underlying mesenchyme.Ladinin is an anchoring filament protein of basement membrane at the dermal-epidermal junction.
Human ladinin is an autoantigen associated with linear IgA disease.
Motoki et al.
(1997) cloned mouse ladinin-1, which encodes a 528aa polypeptide that contains a secretory signal sequence, several putative glycosylation sites, and 6 SEK motifs which may participate in macromolecular interactions.
Northern blotting revealed that the approximately 2.5-kb mouse Lad1 mRNA is expressed in keratinocytes but not in fibroblasts.
It is also expressed in a variety of mouse tissues.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human LAD1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Glu192 of human LAD1, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
