84-4424-91　［受注停止］LACRT (Lacritin, Extracellular glycoprotein lacritin) 200ul　363888

特徴

• Lacritin is a 12.3kD glycoprotein encoded in humans by the LACRT gene.
• Lacritin is a secreted protein found in tears and saliva.
• Lacritin also promotes tear secretion and proliferation of some epithelial cells.
• Lacritin is thus a prosecretory mitogen.
• Most lacritin is produced by the lacrimal gland.
• Some lacritin is produced by the meibomian gland, and also by epithelial cells of the conjunctiva and cornea.
• Together these epithelia comprise much of the lacrimal functional unit (LFU).
• Dry eye is the most common disease of the LFU.
• Preliminary studies with small trials suggest that lacritin may be differentially downregulated in dry eye.
• Topical lacritin promotes tearing in rabbit preclinical studies.
• Lacritin cell targeting is dependent on the cell surface heparan sulfate proteoglycan syndecan-1 (SDC1).
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-E DASSDSTGAD PAQEAGTSKP NEEISGPAEP ASPPETTTTA QETSAAAVQG TAKVTSSRQE LNPLKSIVEK SILLTEQALA KAGKGMHGGV PGGKQFIENG SEFAQKLLKK FSLLKPWA
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.