The protein CD80 is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival.
It is also known as B7.1.
Its principal mode of action is by binding to CD28.
Along with CD86, these molecules provide the necessary stimuli to prime T cells against antigens presented by antigen-presenting cells.
CD80 and CD86 also bind to CTLA-4, a cell surface molecule expressed on activated T cells.
Interactions between CD80 or CD86 with CTLA-4 decrease the response of T cells.
In a nutshell, reactive oxygen stimulates dendritic cells, which activate other immune cells to up-regulate production of CD80, the molecule co-responsible for T cell activation.
"When this pathway is activated, it leads to increased T cell TNF production and ultimately to bone loss."
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-3ug/mlImmunohistochemistry: 5-30ug/mlImmunocytochemistry: 5-30ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse LAB7-1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Asp39-Asn246 of mouse LAB7-1 expressed in E. coli.
