As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails.
The predicted 495aa HB1 protein has an 88aa C-terminal domain.
The aa sequences of human and sheep HB1 are 90% identical.
Northern blot analysis detected HB1 transcripts in human scalp but not unhaired breast epidermis
HB1 is more closely related to HB3 and HB6 than to HB5.
In situ hybridization studies demonstrated that type II keratin genes are sequentially activated in the hair follicle in the following order: HB5--(HB1, HB3)--HB6.
Both HB1 and HB3 mRNA syntheses begin 10 to 15 cell layers above the apex of the dermal papilla and end abruptly in the middle of the cortex.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human KRT81.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser266-Gly422 of human KRT81fused to His-Tag at N-terminal expressed in E. coli.
