Specific pairs of heterotypic keratin chains are coexpressed during the various stages of epithelial differentiation.
This pattern of expression of cytokeratin pairs implies the existence of highly coordinated control of transcription.
The gene for cytokeratin 3 (KRT3), a member of the type II subfamily, is expressed together with KRT12 during 'corneal-type' differentiation (Schermer et al., 1986)
The KRT3 gene is approximately 6.5kb long and encodes one of the largest cytokeratin proteins (64 kD).
By fluorescence in situ hybridization, Raimondi et al.
(1994) mapped the KRT3 gene to 12q12-q13.
All type II keratin genes mapped to that time had been assigned to chromosome 12 where they appeared to be organized into one homotypic cluster.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human KRT3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu198-Ser514 of human KRT3 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
