It is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin.
Together they form the cytoskeleton of epithelial cells.
Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex and Dermatopathia pigmentosa reticularis, both of which are autosomal dominant mutations
KRT14 belongs to a large group of acidic type I keratins that interact with basic type II keratins to form the 8-nm cytoskeletal filaments of epithelial cells.
Both type I and type II keratins have a central alpha-helical domain of over 300aa that mediates keratin interaction.
KRT14 is expressed in the basal layer of stratified squamous epithelia, including epidermis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat KRT14.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Glu122-Glu429 of rat KRT14 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
