The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size.
Small molecules (up to 70kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process.
Most nuclear proteins contain short basic aa sequences known as nuclear localization signals (NLSs)
KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human.
The predicted aa sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively.
The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human KPNa3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu3-Val220 of human KPNa3 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
