Krueppel-like factor 5 is amember of the Kruppel-like factor subfamily of zinc finger proteins.
Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor
Klf5, also known as (IKLF) or (Bteb2) has been assigned purely transcriptional activation activity (Fig.
1A) but, similar to Klf4, binds p300 which acetylates the first zinc finger conferring a trans-activating function.
Importantly for Klf4 & Klf5, the amino acids that are predicted by the Klevit model to interact with DNA are identical (Fig.
1B and Fig.
2) and the two compete for the same CACCC element found in a wide variety of promoters.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human KLF5.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asp216-Asn457 of human KLF5 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
