KLb encodes a 1043aa protein that shares 41.2% identity with mouse Kl.
Like Kl, Klb contains an N-terminal signal sequence, followed by 2 tandemly repeated glycosidase-like domains, a C-terminal transmembrane domain, and a short cytoplasmic tail.
Both Kl and Klb lack 2 key glutamates in the predicted active centers of the glycosidase-like domains
Northern blot analysis of adult mouse tissues showed that Klb was predominantly expressed in liver and pancreas, with lower levels in skin and weak expression in stomach, skeletal muscle, small intestine, and lung.
In mouse embryos, Klb expression became detectable at day 11 and increased in intensity as development progressed.
In situ hybridization of mouse embryos revealed strong Klb expression in yolk sac, gut, brown and white adipose tissue, liver, and pancreas.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse KLb.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ser380-Phe506 of mouse KLb fused to His-Tag at N-terminal expressed in E. coli.
