Kidney injury molecule-1 (KIM-1) is a type 1 transmembrane protein that is not detectable in normal kidney tissue but is expressed at high levels in rodent kidneys with dedifferentiated proximal tubule epithelial cells after ischemic or toxic injury
Sequence analysis predicted that the 359aa type I HAVCR1 glycoprotein, which is 79% identical to the monkey protein, contains a signal sequence; a 109aa cys-rich N-terminal region with only 1 conserved N-glycosylation site; a 163aa TSP-rich region with 3 N-glycosylation sites, 2 of which are conserved; and a 48aa intracellular domain, 12 residues shorter than that of the monkey protein.
Northern blot analysis revealed ubiquitous expression of a 4.4-kb HAVCR1 transcript, with highest levels in kidney and testis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry (Frozen): 5-20ug/mlImmunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher.
Included Protein Molecular Weight Marker:168631: Unstained Protein Molecular Weight Marker, 10-70kDSuppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol.
Ready to use; no need to heat, dilute or add reducing agents before use.
Protein Bands: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD
Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier
Usage: Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20°C will go back into solution.
Do not boil! Load the following volumes on SDS-PAGE gel:Mini gels: 3-5ul/wellLarge gel: 7ul/wellThe loading volumes listed above are recommended for gels with thickness of 0.75-1mm.
The loading volume should be doubled for 1.5mm thick gels.
Store at -20°C Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：porcine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Specificity：Recognizes porcine Kim1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Po
Immunogen：Recombinant protein corresponding to Val5-Val107 of porcine Kim1, expressed in E. coli.
