Kidney injury molecule-1 (KIM-1) is a type 1 transmembrane protein that is not detectable in normal kidney tissue but is expressed at high levels in rodent kidneys with dedifferentiated proximal tubule epithelial cells after ischemic or toxic injury
Sequence analysis predicted that the 359aa type I HAVCR1 glycoprotein, which is 79% identical to the monkey protein, contains a signal sequence; a 109aa cys-rich N-terminal region with only 1 conserved N-glycosylation site; a 163aa TSP-rich region with 3 N-glycosylation sites, 2 of which are conserved; and a 48aa intracellular domain, 12 residues shorter than that of the monkey protein.
Northern blot analysis revealed ubiquitous expression of a 4.4-kb HAVCR1 transcript, with highest levels in kidney and testis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Kim1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser21-Ala240 of human Kim1, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
