Kinesins are microtubule-based motor proteins involved in the transport of organelles in eukaryotic cells.
They The predicted 963aa protein has 63% sequence identity to the Drosophila KHC.
Immunoblot analysis using antibodies against squid KHC detected a 120kD protein in CV-1 monkey kidney epithelial cells.
Immunoblot analysis using antibodies against squid KHC detected a 120kD protein in CV-1 monkey kidney epithelial cells.
Immunofluorescence studies showed that KNS1 expressed in CV-1 cells had both a diffuse distribution and a filamentous staining pattern that coaligned with microtubules but not vimentin (VIM) intermediate filaments; the KNS1 N- and C-terminal domains, but not the alpha-helical rod domain, also colocalized with microtubules.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat KIF5B.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ile9-Lys226 of rat KIF5B fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
