KIF18A contains a motor domain and conserved ATP- and microtubule-binding sites, and the central region is predicted to have an alpha-helical coiled-coil, followed by a C-terminal cargo-binding domain.
Nuclear localization signals, protein-protein interaction motifs such as SH3, WW, AP-1, and FHA domains, and potential sites for phosphorylation and sumoylation were also identified.
KIF18A shares 84% and 81% homology with the mouse and rat homologs, respectively.
Indirect immunofluorescence microscopy localized endogenous KIF18A to the nucleus, cytoplasm, and plasma membrane ruffles in preosteogenic marrow stromal cells.
Punctate cytoplasmic staining of KIF18A was associated with microtubules and partial colocalization with actin.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human KIF18A.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Lys453 of human KIF18A fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
